BrightLine®單波段和多波段LED濾光片組 
BrightLine®單波段和多波段LED濾光片組 Semrock從今天市場上受歡迎的led光源的光譜開始，設(shè)計這個濾光片系列。與傳統(tǒng)的用于標(biāo)準(zhǔn)寬頻光源(如水銀或氙氣燈)的傳統(tǒng)濾光片組相比，這些濾光片可顯著改善熒光信號。 這些濾光片組被優(yōu)化，以同時傳送亮的信號和高信噪比(對比度)，可用于使用基于LED的光引擎成像各種熒光團。 光譜與流行的LED光引擎對齊 與傳統(tǒng)光源相比，在使用led光引擎時，每個通道的信號比傳統(tǒng)光源多10倍到100倍 該系列中的常見激發(fā)濾光片簡化了不同設(shè)置配置的可視化 BrightLine Single-band LED Filter Sets BrightLine單波段LED濾光片組 濾光片/主要熒光 | 激發(fā)(CWL/BW) | 發(fā)射 (CWL/BW) | 二向色邊緣 | 濾光片組零件號/ 零組零件編號 | LED-DAPI-A DAPI, Alexa Fluor® 405, BFP, Pacific BlueTM | 392/23 | 447/60 | 409 nm | LED-DAPI-A-000 LED-DAPI-A-000-ZERO | LED-CFP-A CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | 438/24 | 483/32 | 458 nm | LED-CFP-A-000 LED-CFP-A-000-ZERO | LED-FITC-A FITC (Fluorescein), GFP (EGFP), Cy2TM, 5-FAM | 474/27 | 525/45 | 495 nm | LED-FITC-A-000 LED-FITC-A-000-ZERO | LED-YFP-A YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | 509/22 | 544/24 | 526 nm | LED-YFP-A-000 LED-YFP-A-000-ZERO | LED-TRITC-A TRITC, SpectrumOrange, dTomato, mTangerine | 554/23 | 609/54 | 573 nm | LED-TRITC-A-000 LED-TRITC-A-000-ZERO | LED-mCherry-A mCherry, TexasRed, mKate, mRFP1 | 578/21 | 641/75 | 596 nm | LED-mCherry-A-000 LED-mCherry-A-000-ZERO | LED-Cy5-A Cy5TM, Alexa Fluor® 647, APC | 635/18 | 680/42 | 652 nm | LED-Cy5-A-000 LED-Cy5-A-000-ZERO |
“平克爾”多頻帶LED濾光片組- 單頻激發(fā)器，一個三或四波段發(fā)射器，和一個多分束器 濾光片/主要熒光 | 多通帶激發(fā) | 多通帶發(fā)射 | 多邊緣二向色 | 濾光片組零件號 | LED-DA/FI/TR/Cy5-A (Quad-band Full Multiband) Blue: DAPI, BFP, Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | 392/23 474/27 554/23 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-A-000 LED-DA/FI/TR/Cy5-A-000- ZERO |
“Sedat”多通帶LED濾光片組-單頻帶激發(fā)器和發(fā)射器，以及一個多邊分束器 濾光片組/主要熒光 | 單波段激發(fā) | 多通帶發(fā)射 | 多邊緣二向色 | 濾光片組零件號 | LED-DA/FI/TX-3X-A (Triple-band Pinkel Set) | | | | LED-DA/FI/TX-3X-A-000 | Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 | Ex1: 392/23 | 432/36 | 409 nm | LED-DA/FI/TX-3X-A-000-ZERO | Green: FITC (Fluorescein), GFP (EGFP),Cy2TM | Ex2: 474/27 | 523/46 | 493 nm | | Red: Texas Red, mCherry, 5-ROX | Ex3: 575/25 | 702/196 | 596 nm | | LED-CFP/YFP/mCherry-3X-A (Triple-bandPinkelSet) | | | | LED -CFP/YFP/mCherry- | Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | Ex1: 438/24 | 475/22 | 459 nm | 3X-A-000 | Yellow: YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | Ex2: 509/22 | 543/22 | 526 nm | LED -CFP/YFP/mCherry-3X-A- | Red: mCherry, TexasRed, mKate, mRFP1 | Ex3: 578/21 | 702/197 | 596 nm | 000-ZERO | LED-DA/FI/TR/Cy5-4X-A (Quad-band Pinkel Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000- ZERO |
“平克爾”多頻帶LED濾光片組- 單頻激發(fā)器，一個三或四波段發(fā)射器，和一個多分束器 濾光片組/主要熒光 | 單波段激發(fā) (CWL/BW) | 單波段發(fā)射 (CWL/BW) | 多邊緣二向色 | 濾光片組編號 | LED-DA/FI/TX-3X3M-A (Triple-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Cy2TM Red: Texas Red, mCherry, 5-ROX | Ex1: 392/23 Ex2: 474/27 Ex3: 575/25 | Em1: 433/24 Em2: 525/45 Em3: 641/75 | 409 nm 493 nm 596 nm | LED-DA/FI/TX-3X3M-A-000 LED-DA/FI/TX-3X-A-000-ZERO | LED-CFP/YFP/mCherry-3X3M-A (Triple-band Sedat Set) Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP Yellow: YFP (EYFP), Eosin, Fluo-3, Rhodamine 123 Red: mCherry, TexasRed, mKate, mRFP1 | Ex1: 438/24 Ex2: 509/22 Ex3: 578/21 | Em1: 482/25 Em2: 544/24 Em3: 641/75 | 459 nm 526 nm 596 nm | LED-CFP/YFP/mCherry- 3X3M-A-000 LED -CFP/YFP/mCherry-3X-A- 000-ZERO | LED-DA/FI/TR/Cy5-4X4M-A (Quad-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | Em1: 433/24 Em2: 520/35 Em3: 600/37 Em4: 680/42 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000-ZERO |
SearchLight允許熒光顯微鏡用戶和光學(xué)儀器設(shè)計者為他們的顯微鏡或系統(tǒng)預(yù)先確定z佳的熒光素、光源、探測器和光學(xué)濾鏡組合。通過消除對光譜數(shù)據(jù)源的猜測和搜索時間，探照燈用戶將能夠消除誤差并更有效的工作。用戶可以從大量的預(yù)先加載的光譜中選擇或者在這個免費且可公開訪問的工具中上傳他們自己的光譜數(shù)據(jù)。用戶也可以安全地保存和共享數(shù)據(jù)。 共享:SearchLight的共享功能允許研究人員、工程師、公司和機構(gòu)之間建立一個*的URL鏈接，可以通過電子郵件發(fā)送給同事或合作者。 立即使用探照燈。節(jié)省時間。試一試在:http://searchlight.semrock.com 
Semrock生產(chǎn)多波段熒光濾光片，具有通帶、邊緣陡度和阻滯性能，可與z佳單波段濾光片相媲美，并且具有硬鍍膜的優(yōu)異“抗燒毀”耐久性。事實上，在每一個BrightLine濾光片組中的濾光片，包括這些多波段的濾光片，都是用同樣的耐用的硬鍍膜技術(shù)制成的。 BrightLine® Multiband Fluorescence Sets BrightLine®多波段熒光濾光片組 濾光片組/主要熒光 | 多波段激發(fā)(CWL/BW) | 多波段發(fā)射(CWL/BW) | 多邊緣二向色 | 濾光片組零件編號 | DA/FI-A (Dual-Band Full Multiband) | | | | DA/FI-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 480/29 | 530/40 | 502 nm | | CFP/YFP-A (Dual-Band Full Multiband) | | | | CFP/YFP-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | 416/25 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | 501/18 | 547/31 | 520 nm | | GFP/DsRed-A (Dual-Band Full Multiband) | | | | GFP/DsRed-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | 468/34 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | 553/26 | 630/91 | 574 nm | | FITC/TxRed-A (Dual-Band Full Multiband) | | | | FITC/TxRed-A-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 479/38 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | 585/27 | 628/33 | 606 nm | | Cy3/Cy5-A (Dual-Band Full Multiband) | | | | Cy3/Cy5-A-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | 534/36 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | 635/31 | 690/50 | 659 nm | | DA/FI/TR-A (Triple-Band Full Multiband) | | | | DA/FI/TR-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/36 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 478/24 | 517/23 | 497 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 555/19 | 613/61 | 574 nm | | DA/FI/TX-B (Triple-Band Full Multiband) | | | | DA/FI/TX-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 407/14 | 457/22 | 436 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 494/20 | 530/20 | 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 576/20 | 628/28 | 604 nm | | DA/FI/TR/Cy5-A (Quad-Band Full Multiband) | | | | DA/FI/TR/Cy5-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 440/40 | 410 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 485/20 | 521/21 | 504 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 559/25 | 607/34 | 582 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 649/13 | 700/45 | 669 nm | |
高的透射率，阻擋度和邊緣陡度，可實現(xiàn)令人眼花繚亂的視覺和數(shù)字成像 硬的、絕緣的鍍膜為每個濾光片，包括UV激發(fā)器的“抗燒毀”性能 光譜復(fù)雜的濾光片是我們的特產(chǎn)。世界上僅有的五種彩色多波段組和大量的四、三、雙波段組可供選擇，也保證有庫存。 “平克爾”多波段濾光片組- 單頻帶激發(fā)器，一種雙-三-四或五波段發(fā)射器和一種多邊分束器 濾光片組/主要熒光團 | 多波段激發(fā)(CWL/BW) | 多波段發(fā)射(CWL/BW) | 多邊緣二向色 | 濾光片組編號 | DA/FI-2X-B (Dual-Band Pinkel Set) | | | | DA/FI-2X-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | Ex1: 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex2: 485/20 | 530/40 | 502 nm | | CFP/YFP-2X-A (Dual-Band Pinkel Set) | | | | CFP/YFP-2X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | Ex2: 504/12 | 547/31 | 520 nm | | GFP/DsRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/DsRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 470/22 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | Ex2: 556/20 | 630/91 | 574 nm | | GFP/HcRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/HcRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex2: 585/29 | 645/49 | 605 nm | | FITC/TxRed-2X-B (Dual-Band Pinkel Set) | | | | FITC/TxRed-2X-B-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex1: 485/20 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | Ex2: 586/20 | 628/33 | 606 nm | | Cy3/Cy5-2X-B (Dual-Band Pinkel Set) | | | | Cy3/Cy5-2X-B-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | Ex1: 534/30 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | Ex2: 628/40 | 690/50 | 659 nm | | BFP/GFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | BFP/GFP/HcRed-3X-A-000 | Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor® 350 | Ex1: 370/36 | 425/35 | 395 nm | | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex2: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 585/29 | 685/130 | 610 nm | | CFP/YFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | CFP/YFP/HcRed-3X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 444 nm | | Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123 | Ex2: 504/12 | 542/27 | 521 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 589/15 | 639/42 | 608 nm | | DA/FI/TR-3X-A (Triple-Band Pinkel Set) | | | | DA/FI/TR-3X-A-000 | Blue: DAPI | Ex1: 387/11 | 433/36 | 403 nm | | Green: FITC (Fluorescein), GFP (EGFP) | Ex2: 480/17 | 517/23 | 497 nm | | Orange: TRITC (Tetramethylrhodamine) | Ex3: 556/20 | 613/61 | 574 nm | | DA/FI/TX-3X-C (Triple-Band Pinkel Set) | | | | DA/FI/TX-3X-C-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, BoDipy, Alexa Fluor® 488 | Ex1: 387/11 Ex2: 494/20 | 457/22 530/20 | 436 nm 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | Ex3: 575/25 | 628/28 | 604 nm | | DA/FI/TR/Cy5-4X-B (Quad-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 | 440/40 521/21 607/34 700/45 | 410 nm 504 nm 582 nm 669 nm | DA/FI/TR/Cy5-4X-B-000 | DA/FI/TR/Cy5/Cy7-5X-A (Penta-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 Deep Red: Cy7™ | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 Ex5: 740/13 | 440/40 521/21 607/34 694/35 809/81 | 408 nm 504 nm 581 nm 667 nm 762 nm | DA/FI/TR/Cy5/Cy7 -5X-A-000 |
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